Aflatoxin - Total (AFLA) Elisa Test Kit

Product Code AFLA-0050
Kit contains approximately 42 test samples plus controls, contains 6 microtiter test strips.

Aflatoxins are toxic metabolites produced by a variety of molds such as Aspergillus flavus and Aspergillus parasiticus. They are carcinogenic and can be present in grains, nuts, cottonseeds and other commodities associMated with human food or animal feeds. Crops may be contaminated by one or more of the four following sub-types of aflatoxin: B1, B2, G1 and G2. Aflatoxin B1 is the most toxic and frequently detected form. The other types present a significant danger if the concentration is at a high level. Aflatoxins have been implicated in human health disorders including hepatocellular carcinoma, aflatoxicosis, Rey’s syndrome and chronic hepatitis. Animals are exposed to aflatoxins by consumption of feeds that are contaminated by aflatoxin producing fungal strains during growth, harvest or storage. Symptoms of toxicity in animals range from death to chronic diseases, reproductive interference, immune suppression, decreased milk and egg production. Most controlling government agencies worldwide have regulations regarding the amount of aflatoxins allowable in human and animal foodstuffs. Accurate and rapid determination of the presence of aflatoxin in commodities is of paramount importance.

Warning: Toxic mold can cause serious, permanent, and disabling health problems. Some species of mold release mycotoxin. Mycotoxins are substances that may be toxic to humans and can cause serious systemic injury. Examples of mycotoxins are Aflatoxins from Aspergillus flavus, Trichothecenes from Stachybotrys arta, and Ocratoxin from Penicillium cyclopium.

PRINCIPLE OF ASSAY

An aflatoxin specific antibody optimized to react with B1 is coated to a polystyrene microwell. The extract of grain is added to the microwell and incubated in competition with a conjugated aflatoxin B1 antigen. Any aflatoxin B1 antigen present in the sample will bind to the -aflatoxin B1 on the microwell forming an aflatoxin B1 antibody-antigen complex. After washing away any unbound material, enzyme substrate is then added to the microwell. The substrate reacts with any bound enzyme conjugate present and forms a blue product. In the final step the reaction is stopped, changing the color from blue to yellow, fixing the color. The intensity of the color is measured photometrically at 450 nm.