Deoxynivalenol - Vomitoxin (DON) Elisa Test Kit

Product Code DON-0050
Kit contains approximately 42 test samples plus controls, contains 6 microtiter test strips.

Deoxynivalenol (DON) is a low molecular weight metabolite of the tricothecene mycotoxin group produced by fungi of the Fusarium genus, particularly F. graminearum. These fungi occur widely and will infect barley, wheat, and corn (maize). Deoxynivalenol is highly toxic, producing a wide range of immunological disturbances and is particularly noted for inducing feed refusal and emesis in pigs, hence the alternative name vomitoxin.

PRINCIPLE OF ASSAY

The Deoxynivalenol (DON) Assay is a solid phase direct competitive enzyme immunoassay. A deoxynivalenol specific antibody is coated to a polystyrene microwell. Toxins are extracted from a ground sample with distilled or deionized water. The extracted sample and HRP-conjugated DON are mixed and added to the antibody-coated microwell. DON from the extracted sample and HRP-conjugated DON compete to bind with the antibody coated to the microwell. Microwell contents are decanted and non-specific reactants are removed by washing. An enzyme substrate (TMB) is added and color (blue) develops. The intensity of the color is directly proportional to the amount of bound conjugate and inversely proportional to the concentration of DON in the sample or standard. Therefore, as the concentration of DON in the sample or standard increases, the intensity of the blue color will decrease. An acidic stop solution is added which changes the chromagen color from blue to yellow. The microwells are measured optically by a microplate reader with an absorbance filter of 450nm (OD450). The optical densities of the samples are compared to the OD’s of the kit standards and an interpretative result is determined.