Infectious Laryngotracheitis Virus Antibody Test Kit

Product Code LTI-1000
Kit includes approximately 920 test samples plus controls, contains 10 microtiter plates.
Product Code LTI-0500
Kit includes approximately 460 test samples plus controls, contains 5 microtiter plates.
Product Code LTI-0200
Kit includes approximately 184 test samples plus controls, contains 2 microtiter plates.

Infectious laryngotracheitis (commonly known as ILT) is a viral respiratory disease of chickens centred on the trachea. ILT can cause serious production losses due to a combination of mortality and depressed egg production. This disease can manifest itself in a variety of forms ranging from severe to mild with the latter being increasingly encountered in developed poultry industries and appearing as a mucoid tracheitis, sinusitis and conjunctivitis coupled to unthriftiness and low mortality.

INTENDED USE
ELI-LTI ELISA kit is used for quantitative detection of antibodies against Infectious Laryngotracheitis virus (ILT) in chicken serum and yolk.

Inactivated antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-ILT antibodies present in the serum will bind to the AIV on the microwell forming an AIV antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the ILT antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 - 410 nm with a 630-650 nm reference filter.