Ochratoxin A (OCH) Elisa Test Kit

Product Code OCH-0050
Kit contains approximately 42 test samples plus controls, contains 6 microtiter test strips.

Ochratoxin A is a toxic metabolite produced by several molds of the Aspergillus flavus and Penicillium genera, including Aspergillus ochraceus. The fungal species has the potential to produce ochratoxin A, a known nephrotoxin and carcinogen. It has been frequently detected in human foods and animal feed, mainly in cereal products, although a range of commodities has been reported to contain the toxin. In humans, exposure to ochratoxin A has been linked with Balken endemic nephropathy (BEN), a chronic kidney disease associated with tumors of the renal system. In animals, impairment of renal function has been reported in swine. In turkeys and chickens symptoms included retarded growth, decreased feed conversion, nephropathy and mortality. Feed refusal has also been observed in turkeys. A decrease in egg production and shell quality was reported in both turkeys and chickens.

PRINCIPLE OF ASSAY

The Ochratoxin A Assay is a solid phase direct competitive enzyme immunoassay. An Ochratoxin specific antibody optimized to cross react primarily with Ochratoxin A (see cross-reactivity information) is coated to a polystyrene microwell. Toxins are extracted from a ground sample with 70% methanol. The extracted sample and HRP-conjugated Ochratoxin are mixed and added to the antibody-coated microwell. Ochratoxin from the extracted sample and HRP-conjugated Ochratoxin compete to bind with the antibody coated to the microwell. Microwell contents are decanted and non-specific reactants are removed by washing. An enzyme substrate (TMB) is added and color (blue) develops. The intensity of the color is directly proportional to the amount of bound conjugate and inversely proportional to the concentration of Ochratoxin in the sample and standards. Therefore, as the concentration of Ochratoxin in the sample or standard increases, the intensity of the blue color will decrease. An acidic stop solution is added which changes the chromagen color from blue to yellow. The microwells are measured optically by a microplate reader with an absorbance filter of 450 nm (OD450). The optical densities of the samples are compared to the OD’s of the kit standards and an interpretative result is determined.